The Purine Nucleotide Cycle
نویسندگان
چکیده
منابع مشابه
The Purine Nucleotide Cycle
Linked oscillations of the glycolytic pathway and the purine nucleotide cycle were studied in particle-free extracts of rat skeletal muscle. Under the conditions used, an accumulation of about 1 pM fructose diphosphate can trigger a sudden increase in phosphofructokinase activity. The activation by fructose diphosphate depends on the presence of AMP. When the AMP concentration drops, phosphofru...
متن کاملThe purine nucleotide cycle and ammoniagenesis in rat kidney tubules.
The contribution of the purine nucleotide cycle to renal ammoniagenesis was examined in cortical tubule suspensions prepared from acidotic rats and incubated with [alpha-15N]glutamine, [15N]glutamate, or [15N]aspartate. Labeling of ammonia and adenine nucleotides was determined after enzymatic transformations designed to circumvent the technical problem that 15NH3 and H2O have the same nominal ...
متن کاملVitamin C deficiency activates the purine nucleotide cycle in zebrafish.
Vitamin C (ascorbic acid, AA) is a cofactor for many important enzymatic reactions and a powerful antioxidant. AA provides protection against oxidative stress by acting as a scavenger of reactive oxygen species, either directly or indirectly by recycling of the lipid-soluble antioxidant, α-tocopherol (vitamin E). Only a few species, including humans, guinea pigs, and zebrafish, cannot synthesiz...
متن کاملThe purine nucleotide cycle. The production of ammonia from aspartate by extracts of rat skeletal muscle.
Extracts of rat skeletal muscle convert aspartate to ammonia and fumarate plus malate. The conversion is dependent on the presence of catalytic amounts of IMP, adenylosuccinate, or AMP. In addition, GTP is required as a source of energy. A GTP-regenerating system must be supplied since the accumulation of GDP inhibits the operation of the cycle. It is proposed that the purine nucleotide cycle a...
متن کاملThe purine nucleotide cycle. A pathway for ammonia production in the rat kidney.
Particle-free extracts prepared from kidney cortex of rat catalyze the formation of ammonia via the purine nucleotide cycle. The cycle generates ammonia and fumarate from aspartate, using catalytic amounts of inosine monophosphate, adenylosuccinate, and adenosine monophosphate. The specific activities of the enzymes of the cycle are 1.27+/-0.27 nmol/mg protein per min (SE) for adenoylosuccinate...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)44058-1